fast blue b salt test principle

C07C245/00 Alkylresorcinols are phenolic lipid compounds that are present in food almost exclusively in bran and whole-grain products based on wheat and rye. Wieland, private communication). <> In most procedures, silyl derivatives of alkylresorcinols are prepared to improve the volatility of the compounds. 196 0 obj Many of these chemicals are under eco-pressure (Chattopadhyay and Kumar, 2001). Seq Text D.P. The whole procedure is performed in a plastic pouch by visual inspection. These alkaloids have been found in a surprising range of plants. Contains list of applicants of the patent document Bag Text This last reagent is the most preferred and was shown to be the most sensitive for the visualization of ARs (Iliev et al., 2009). patent Contains list of inventors of the patent document IL Use of 96 well plates made possible the extraction of 160 samples each day. C.I. - B310 for a priority Narcopouch (ODV Inc., Paris, ME, USA) is a battery of color tests for the detection of opiates, amphetamines, cocaine, barbiturates, cannabinoids, and lysergic acid diethylamide (LSD) in street samples. Developing agents are all diazotisable amines or diamines or substituted anilines, toluidines, anisidines, azobenzenes or diphenylamines. Contains abstract or an ordered list of abstracts of the patent document - XML ST36 element: Using a 5cm NH2-column and heptanes decreased run times to 3min. 1983-07-25 CAED was the most sensitive followed by FD and UV. Patent Bibliographic Data Schema V. 1.0 - B140/date for a publication or B871/date for an international publication Provides a structure for document identification related data Reagents, test kits and methods for the detection of cannabinoids - European Patent Office - EP 0132313 B1 The majority of the active ingredients of drug powders that are encountered in forensic examinations are basic in nature and may therefore be examined using one of the general systems described in the article on Toxicological Analysis. Besides these fast bases there are other bases or their salts which are also harmful and should not be used; these include Fast Yellow GC Base (http://actrav.itcilo.org/actrav-english/telearn/osh/ic/95512.htm [accessed 16 February 2010]), Fast Red A1 salt (http://msds.chem.ox.ac.uk/FA/fast_red_A1_salt.html [accessed 16 February 2010]), Black K salt (http://msds.chem.ox.ac.uk/FA/fast_black_k_salt.html [accessed 16 February 2010]) and Fast Red violet diazonium salt (Woods and Ellis, 1994). AMD showed higher resolution exhibiting neither diffusion nor spot stretching. OPLC offered a higher efficiency, reproducibility, smaller consumption of developing eluent, and shorter analysis delay as well as the possibility of working in a semipreparative way [76]. http://www.epo.org/patent-bibliographic-data/1.0/ pages) Korte et al. In this case a general screen for acidic and neutral drugs, as described in the article on TLC in analytical toxicology, would be carried out. Peterson et al. http://www.epo.org/patent-bibliographic-data/1.0/ B1 - B320/date for a priority While investigating the aloe exudate compounds by TLC, a zone staining yellow with Fast Blue B (0.5% aqueous solution) was noted in a few species. xapMM Schema external Fischedick et al. uuid:ec1a2cf6-1dd1-11b2-0a00-841e42080100 URI InternationalApplication Marquis, Meyer's, Mecke, Ehrlich's, FORENSIC SCIENCES | Thin-Layer Chromatography, Encyclopedia of Analytical Science (Second Edition), ml)), which, after overspraying with hydrochloric acid, gives blue to violet colors with cannabinoids. endobj endobj external EP0132313 TLC may be used for samples, such as stomach contents and urine, that contain the greatest amount of drugs and their metabolites, but the levels found in blood are usually low and preclude the use of TLC. V)gB0iW8#8w8_QQj@&A)/g>'K t;\ $FZUn(4T%)0C&Zi8bxEB;PAom?W= endobj 37105), Fast Red GTR Base (Azoic Diazo Component 29, C.I. Publication 69075 Recently, Sampietro, Jimenez, Belizn, Vattuone, and Cataln (2013) developed a micro method for the quantification of 5-n-alkylresorcinols in grain and grain products. endobj C07C245/00 The reductive enzymes present in the body such as NaDPH-cytochrome reductase and cytochrome-450 can reduce the azo and amino groups. Date The second consists of a mobile phase of toluene in an open tank and a silica gel plate sprayed with a 10% solution of silver nitrate and dried before use. Maciej J. Bogusz, in Handbook of Analytical Separations, 2008. - B330/ctry for a priority Contains list of Patent Classification symbols of the patent document - XML ST36 element: 1 Contains list of proprietors of the patent document Seq Bookmark Later, -coniceine and coniine were again isolated from A. sabaea, together with a new compound N,N-dimethylconiine as a minor component (Blitzke et al., 2000). Chromatographic separation using HPLC and GC with UV, fluorescence (FD), Coulombic array electrochemical detection (CAED), and MS detection methods has been used for the analysis of alkylresorcinols. (=A. gillilandii Reynolds) (Dring et al., 1984). N')].uJr A general location reagent is Duquenois reagent (vanillin (2g) and acetaldehyde (0.3ml) in ethanol (100ml)), which, after overspraying with hydrochloric acid, gives blue to violet colors with cannabinoids. Real Provides a structure for describing bookmarks of a patent document The Herosol (Mistral Detection Ltd., Jerusalem, Israel) field kit consists of a spray reagent and special test paper. Kulawinek and Kozubek (2008) reported that the most general methods used for the determination of total AR content are based on spectrophotometry. Fig. 9 StartPage G01N 33/94 The improved GC-MS technique presented could be used for rapid analysis of alkylresorcinols in relatively small sample volumes (Landberg et al., 2009). The authors also pointed out that UV detection generally resulted in an over-estimation of alkylresorcinols concentration. <> The sample may consist of a powder and/or tablets found in the possession of an individual, it may originate from a much larger seizure of material, for example, a consignment being smuggled across international borders, or may be from a number of samples taken from the site of manufacture of the drug, i.e., an illicit laboratory. Following the German ban in 1994, several other European community countries (EU member states) introduced similar regulations. Classification No analytical procedure is currently available for 4-aminoazobenzene. They are being suggested to be used as selective biomarkers of wheat, rye, and whole-grain intake. Adobe PDF Library 8.0 Contains an ordered list of bookmark-related data (name, number of the first page, number of A longer color permanence was observed when the chromogenic reactive was sprayed after the evaporation of surplus solvent, but before total volatilization. This reagent reacts with both phenols and amines, so eventually the latter was pursued leading to the isolation of -coniceine as its hydrochloride from A. sabaea Schweinf. These may include drugs of abuse, as well as those prescribed for medicinal use and a number of other miscellaneous poisons. Number InternationalPublication 15 For drugs analyses the major applications of TLC are concerned with the identification of cannabis and the screening of drug powders and tablets for their active constituents. Drug Wipe and Drug Wipe II (Securetec AG, Germany) are immunochemical tests designed for the detection of drugs of abuse on different surfaces, e.g. Landberg, Aman, and Kamal Eldin (2009) used an OasisMax solid phase extraction cartridge for sample cleanup to develop a rapid GCMS method for the quantification of alkyl resorcinols in human plasma. Samples in the range of 78750nmol were successfully analyzed. DocId 2y.-;!KZ ^i"L0- @8(r;q7Ly&Qq4j|9 Country Code - XML ST36 element: Coniine, conhydrinone and conhydrin were also found in some of the species (Dring et al., 1984; Nash et al., 1992) (Table 1). Initially the modification was due to the unavailability of the Fast Blue BF4 reagent that was replaced by Fast Blue B ZnCl2 salt that was commercially available. Advances in HPLC that allow higher back pressure (>1000bar) and smaller particle size columns (<3m) provide better resolution of ARs. Hence the workers using harmful amines in the textile industry or dye manufacturing units are at high risk. Text The colorimetric method of detection and quantification is simple, reliable, and inexpensive. wG xR^[ochg`>b$*~ :Eb~,m,-,Y*6X[F=3Y~d tizf6~`{v.Ng#{}}jc1X6fm;'_9 r:8q:O:8uJqnv=MmR 4 %PDF-1.4 % NumberOfPages Thin layer chromatography (TLC) is a suitable method for screening different kinds of samples. claims Therefore, these tests may be used for the detection of drugs in sweat or saliva. Workers engaged in diazotisation of these amines are exposed to the flying dust of these carcinogenic chemicals. Contains number and filing date of the international patent document (2012) developed a HPLC method for the separation of alkylresorcinols from 15 cereal grains and 90 cereal products and compared the performance of three different detectors (UV, FD, and CAED). Many, however, do not, and in these circumstances the scope of the investigation needs to be widened to include the large number of other substances that may be legitimately prescribed but which are nevertheless subject to abuse in some way. The colorimetric method based on diazonium salt, Tluscik, Kozubek, and Mejbaum-Katzenellenbogen (1981), Sampietro, Jimenez, Belizn, Vattuone, and Cataln (2013), Hemlock alkaloids from Socrates to poison aloes, ) but none of these substances are toxic. external The limit of quantification and detection for the three methods were significantly different. They carried out method validation by determining the limits of quantification and detection (LOQ and LOD), the linear dynamic range, and the repeatability. In drugs cases the compound(s) of interest may usually be defined readily; toxicology cases may be complicated by the presence of the many naturally occurring compounds in the body and the presence of drug metabolites, which in some cases are found to the total exclusion of the parent drug. List of banned aromatic amines according to the EU Directive 2002/61/EC. Title Maria John, in Journal of Functional Foods, 2015. description Bag Text 3). [<>] These plants from Africa and Arabia do not generally contain poisons, although a rat-like odour was recorded for Aloe ballyi Reynolds (Reynolds, 1966). EP 0132313 This rapid method correlated well (R2=0.9944) with the commonly used Fast Blue B method and absorbance measurement of colored azo complex at 520nm. Amines 5 and 6 are analysed indirectly via reduction to the amines 18 and 19, respectively. DocId n3kGz=[==B0FX'+tG,}/Hh8mW2p[AiAN#8$X?AKHI{!7, Reagents, test kits and methods for the detection of cannabinoids, Reagents, test kits and methods for the detection of cannabinoids - European Patent Office - EP 0132313 B1, Reagenzien, Teststze und Verfahren zum Nachweis der Kannabinoide, Ractifs, trousses de ractifs et mthodes pour la dtection des cannabinoides. The colorimetric method of detection and quantification is simple, reliable, and inexpensive. A violet color indicates the presence of heroin. nQt}MA0alSx k&^>0|>_',G! Narcopouch (ODV Inc., Paris, ME, USA) is a battery of color tests for the detection of opiates, amphetamines, cocaine, barbiturates, cannabinoids, and lysergic acid diethylamide (LSD) in street samples. The testing devices are simple and robust, and usually based on well-known color reactions. The micro-colorimetric method has been reported for quantitative determination of ARs in plant material (Tucik et al., 1981). Kind Code - XML ST36 element: B130 for a publication or B871/kind for an international publication 194 0 obj Application Marquis, Meyer's, Mecke, Ehrlich's, Fast Blue B, and Koppanyi. Applicant They validated this method and compared it with GC-MS. Some HPLC methods have longer run times due to poor resolution between the unsaturated homologs and saturated side chains. - B210 for an application or B861/dnum/anum for an international application Thus, they may serve as a complement/alternative to commonly used methods of dietary assessment in epidemiological studies. It can be easily performed in routine tests, and several commercial sets are available on the market for cannabinoid testing [67]. IL Copyright 2022 Elsevier B.V. or its licensors or contributors. In forensic science, drugs are encountered in two specific sets of circumstances, each of which requires quite different investigative procedures. This technique works by using Oasis MAX solid phase extraction cartridges for the cleanup of sample. In the second set of circumstances the toxicological investigation drugs are encountered after ingestion by an individual. The content of alkylresorcinols was estimated using calibration curve (110mg) prepared for pure rye 5-n-pentadecylresorcinol as the reference compound (Zarnowski and Suzuki, 2004). Ardrey, in Encyclopedia of Analytical Science (Second Edition), 2005. en Bag DocId external 200 0 obj 37165) (Aspland, 1997; http://ns.ist.cmu.ac.th/riseat/documents/a_dyes.pdf). Galand et al. 3. This type of investigation is usually characterized by a large amount, in analytical terms, of material that is available for analysis; this is usually in the milligram range, but often kilograms of sample are accessible. Correction Code - XML ST36 element: B151+B132EP - See WIPO ST50 for correction code definition This test uses color reactions with several reagents, e.g. classification-ipcr/text, or B511, B512 - See WIPO ST8 for content structure description A number of the tablets that are encountered during the examination of illicit drugs contain the same active ingredients as the drug powders described above. 37085), Fast Scarlet G Base (Azoic Diazo Component 12, C.I. skin) is wiped with the paper, which is then sprayed with Herosol. <>stream x- [ 0}y)7ta>jT7@t`q2&6ZL?_yxg)zLU*uSkSeO4?c. R -25 S>Vd`rn~Y&+`;A4 A9 =-tl`;~p Gp| [`L` "AYA+Cb(R, *T2B- external Many Aloe species are intensely bitter due to the presence of anthrone-C-glycosides, notably barbaloin, which give them their purgative properties and many other phenolic compounds have been noted throughout the genus (Reynolds, 1985, 2004) but none of these substances are toxic. De Faubert Maunder used diethylamine as a mordant bleach reagent since its low boiling point allows it to be sprayed as a liquid and diffuse as a vapor through the thin-layer chromatographic support. In 1994, the German government banned the use of some azo dyes that yield, on reduction, any of the twenty arylamines, including benzidine and its disubstituted congeners. However, the pigment on reduction will produce 2,4-diaminoanisole (4-methoxy-m-phenylene diamine), since both the azo and nitro groups of the pigment are reduced to amino groups. 5-nitro-o-anisidine is widely used to produce an azo scarlet pigment. EP-0132313-B1-19910918 A more specific reagent is Fast blue B solution (a freshly prepared 1% solution of Fast blue B salt (diazotized o-dianisidine)), with which cannabidiol gives an orange color, cannabinol a violet color, and 9-tetrahydrocannabinol a red color. Since the number of individual drugs encountered in these types of investigation is relatively small, a better indication of the drug involved may be obtained by use of a more specific location reagent, such as Marquis reagent (a mixture of formaldehyde solution (1ml) with sulfuric acid (9ml)) for alkaloids related to morphine, and/or running control substances on the same plate as the unknown sample. 2-Naphthylamine is known to be a human carcinogen based on sufficient proof of carcinogenicity in humans. Many eluent mixtures have been tested according to the reports in the literature, for example, isooctaneethyl acetateacetic acid (30:10:1, v/v/v) [70]; petroleum etherdiethyl ether (90:10) [71]; acetonemethylene chloridediisopropyl etherhexane (1:1:3:20,v/v/v/v) [72]; toluenechloroformmethanol (100:10:1,v/v/v) [73]; hexanedioxane (90:10, v/v) with double migration [74]; and hexanediethyl ether (80:20, v/v) [75]. During selection of an ACC and azoic diazo component care should be taken and it is advisable to consult Safety Data Sheets for the materials. external Several reasons have been presented on the need for efficient, fast, and sensitive methods to analyze total content of ARs in cereal grains, including the use of ARs as biomarkers of whole grain or measure of ARs in breeding programs of cereal crops. Ractifs, trousses de ractifs et mthodes pour la dtection des cannabinoides The rapid and sensitive gas chromatography mass spectrometry (GC-MS) technique can be used for quantification of alkyl resorcinol homologues C17:0, C19:0, C21:0, C23:0, and C25:0 in the human plasma. (2016) tested five different supported extraction techniques for the extraction of alkylresorcinols from plasma and they also improved a normal phase LC-MS/MS in order to lessen the sample analysis time. Fast Blue B and RR dissolved more slowly in water and imparted a yellow background to the plate. This new method had a limit of detection (1.11.8nmol/L) and limit of quantification (3.56.1nmol/L) in plasma for the different alkyl resorcinol homologues. Spiro, Baruch 1 Total number of pages Bag Text Gas chromatography with MS and flame ionization detections (FID) has been used for the analysis of alkylresorcinols. The most remarkable discovery of coniine alkaloids was made in aloes (Fig. 37230), Fast Orange R Base (Azoic Diazo Component 7, C.I. 37270), Fast Blue BB Base (Azoic Diazo Component 20, C.I. endobj Contains title or an alternative list of titles of the patent document - XML ST36 element: B541 used high-performance TLC (HPTLC) after a simple decarboxylation procedure to convert THCA into 9-THC by means of normal-phase HPTLC plates with an automated spotter and a densitometric and UV scanner. endobj Senior, Janet et al 37155) and Fast Violet B Base (Azoic Diazo Component 41, C.I. "F$H:R!zFQd?r9\A&GrQhE]a4zBgE#H *B=0HIpp0MxJ$D1D, VKYdE"EI2EBGt4MzNr!YK ?%_&#(0J:EAiQ(()WT6U@P+!~mDe!hh/']B/?a0nhF!X8kc&5S6lIa2cKMA!E#dV(kel }}Cq9 Later a hospital survey of poisoning by herbal remedies added another species, A. christianii Reynolds to the hazardous list (Nyazema, 1984). Present methods require larger volumes of solvent like >10mL/sample and have relatively less daily sample throughput. Reagents, test kits and methods for the detection of cannabinoids In no instance was the nature of the poisonous principle suspected. EREZ FORENSIC TECHNOLOGY LTD. Toluene or xylene were tested as mobile phases, based on their adequate low volatility and high hydrophobic properties for intense color development. - B190 for a publication or B871/ctry for an international publication Publication or Application or Priority Date - XML ST36 element: CountryCode R.E. Table 1. 1983-06-27 (1990) published the dermatological effect of Naphthol AS (ACC 2). Representative The powders deposit on the body and also enter the body through inhalation. The accuracy of this methodology makes it attractive for fingerprinting Cannabis since a better separation is achieved than by using traditional TLC for the main neutral cannabinoids [77]. DocId document European Patent Office A suspected surface (e.g. HyTSwoc [5laQIBHADED2mtFOE.c}088GNg9w '0 Jb Identification of a drug may be required to ascertain the cause of a suspicious death, to determine the cause of impairment of function, for example, in a driving case, or determining the involvement of drugs as a contributory factor in a crime such as murder or rape. Contains country code, publication number/date, correction code, and kind of the published DocId Infusions of three Aloe species, A. chabaudii Schnl., A. globuligemma Pole Evans and A. ortholopha Christian & Milne-Redh., which had been drunk as a herbal medicine caused deaths in rural Africa (Drummond et al., 1975). 1 bibliography In this method 5-N alkylresorcinols are detected from ground and intact whole grains using Fast Blue RR ZnCl2 to yield colored azo derivatives that were measured at 490nm with a shorter incubation time (15min). (summarized from Dring et al., 1984; Nash et al., 1992). The first, the drugs investigation, is that in which the possession, illicit manufacture, and/or distribution of drugs of abuse, such as cannabis, heroin, amphetamine, and LSD, are involved.

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